Accurate modelling of the retention behaviour of peptides in gradient-elution hydrophilic interaction liquid chromatography
نویسندگان
چکیده
منابع مشابه
the washback effect of discretepoint vs. integrative tests on the retention of content in knowledge tests
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15 صفحه اولHydrophilic interaction liquid chromatography (HILIC) in proteomics
In proteomics, nanoflow multidimensional chromatography is now the gold standard for the separation of complex mixtures of peptides as generated by in-solution digestion of whole-cell lysates. Ideally, the different stationary phases used in multidimensional chromatography should provide orthogonal separation characteristics. For this reason, the combination of strong cation exchange chromatogr...
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A systematic evaluation of retention behavior of carbohydrates in hydrophilic interaction liquid chromatography (HILIC) was performed. The influences of mobile phase, stationary phase and buffer salt on the retention of carbohydrates were investigated. According to the results, the retention time of carbohydrates decreased as the proportion of acetonitrile in mobile phase decreased. Increased t...
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A major disadvantage of gradient elution in terms of speed results from the need to adequately re-equilibrate the column. This work distinguishes two states of re-equilibration: (1) run-to-run repeatability and (2) full equilibration. We find that excellent repeatability (+/-0.002 min in retention time) is achieved with at most 2 column volumes of re-equilibration whereas full equilibration can...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2020
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2019.460650